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Fail at step 2d in SRM analysis instructions #8
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Huh. I can investigate later today. I'm assuming it doesn't resolve itself when you press "OK"? |
Correct @yaaminiv . I'm testing it out and that is the step that got us stuck. |
http://owl.fish.washington.edu/spartina/DNR_PECAN_Run_1_20170303/Combined-gigas-QC.txt is the file that I used in my DIA analyses as well (nb here) I'm wondering if just changing the file extension may work....? |
@emmats @yaaminiv |
I'm going to troubleshoot this more today and adjust the protocol so it can be reproduced. |
Skyline wants fastas, I'm pretty sure. I don't think this is the problem with the replicates, though. |
There was a FASTA file on the Windows machine I forgot to upload to OWL. It can now be found here. Additionally, in Step 2d, sometimes copying and pasting the sequences in the lefthand window doesn't work. I've updated the instructions so you can import a FASTA file and populate the analyte tree. I've also updated the DIA protocols in RobertsLab/resources with this information (SRM one cites DIA protocol so it doesn't need to be updated). Your issues should be resolved! I updated my workflow accordingly. |
When trying to paste proteome in get the follow error.
The text was updated successfully, but these errors were encountered: