The interventional effect of magnetic nanoparticle-albumnin-2, 2(-')dipyridyl (MNA-DPD) on delayed cerebral
Author(s): WANG Ziwen, GUI Zheng, LU Xuegang, GUO Dong, ZHU Ming, LI Zhen, HAN Xinwei, YU Yaoyu
Pages: 293-296
Year: 2017 Issue: 4
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: MNA-DPD; Delayed cerebral vasospasm; Subarachnoid hemorrhage;
Abstract: Objective The interventional effect of magnetic nanoparticle-albumin-2,2'-dipyridyl (MNA-DPD) on delayed cerebral vasospasm (DCVS) with magnetic field targeting was explored.Methods The experimental animals,100 healthy adult New Zealand rabbits,were randomly divided into 5 groups:dimethyl sulphoxide group (DMSO),magnetic nanoparticle-albumin group (MNA),2,2'-dipyridyl group (DPD),magnetic nanoparticle-albumin-2,2'-dipyridyl group (MNA-DPD),and control group.DCVS models were made by the secondary injection of blood in DMSO group,MNA group,DPD group and MNADPD group.0.01％ DMSO,MNA,DPD and MNA-DPD were administered respectively in each experimental group once every day (50 mg/kg).Focusing magnetic field was guided in MNA group and MNA-DPD group.In 7 d after injection,animals were killed and given perfusion fixation.Basilar arteries were extracted for HE staining,Prussian blue staining and Western Blot test.The expression of proliferating cell nuclear antigen (PCNA),vascular endothelial growth factor (VEGF),c-Myc protein and Tumor protein p53 in the seventh day were measured with Western Blot test.Results At 7 d after injection,HE staining and Prussian blue staining showed obvious vasospasm in DMSO group and MNA group,moderate vasospasm in DPD group and nearly no vasospasm in MNA-DPD group.It was significantly different in MNA-DPD group compared with MNA group,DPD group and DMSO group (P ＜ 0.05).Prussian blue staining showed magnetic nanoparticles were dyed blue in the subarachnoid space of MNA group and MNA-DPD group.Western Blot test indicated high expression of PCNA,P53,c-Myc and VEGF in DMSO group,MNA group and DPD group,while low expression in MNA-DPD group (P ＜ 0.05).Conclusion MNA-DPD injection via lumbar cistern can effectively relieve DCVS in rabbits with magnetic field targeting.
Pages: 293-296
Year: 2017 Issue: 4
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: MNA-DPD; Delayed cerebral vasospasm; Subarachnoid hemorrhage;
Abstract: Objective The interventional effect of magnetic nanoparticle-albumin-2,2'-dipyridyl (MNA-DPD) on delayed cerebral vasospasm (DCVS) with magnetic field targeting was explored.Methods The experimental animals,100 healthy adult New Zealand rabbits,were randomly divided into 5 groups:dimethyl sulphoxide group (DMSO),magnetic nanoparticle-albumin group (MNA),2,2'-dipyridyl group (DPD),magnetic nanoparticle-albumin-2,2'-dipyridyl group (MNA-DPD),and control group.DCVS models were made by the secondary injection of blood in DMSO group,MNA group,DPD group and MNADPD group.0.01％ DMSO,MNA,DPD and MNA-DPD were administered respectively in each experimental group once every day (50 mg/kg).Focusing magnetic field was guided in MNA group and MNA-DPD group.In 7 d after injection,animals were killed and given perfusion fixation.Basilar arteries were extracted for HE staining,Prussian blue staining and Western Blot test.The expression of proliferating cell nuclear antigen (PCNA),vascular endothelial growth factor (VEGF),c-Myc protein and Tumor protein p53 in the seventh day were measured with Western Blot test.Results At 7 d after injection,HE staining and Prussian blue staining showed obvious vasospasm in DMSO group and MNA group,moderate vasospasm in DPD group and nearly no vasospasm in MNA-DPD group.It was significantly different in MNA-DPD group compared with MNA group,DPD group and DMSO group (P ＜ 0.05).Prussian blue staining showed magnetic nanoparticles were dyed blue in the subarachnoid space of MNA group and MNA-DPD group.Western Blot test indicated high expression of PCNA,P53,c-Myc and VEGF in DMSO group,MNA group and DPD group,while low expression in MNA-DPD group (P ＜ 0.05).Conclusion MNA-DPD injection via lumbar cistern can effectively relieve DCVS in rabbits with magnetic field targeting.
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