Analysis of lmmunophenotypic features of childhood acute myeloid leukemia
Author(s): Wang Xiaohui, Chen Fang, Fu Shuang, Hu Yanping, Zhang Minyu, Liu Xuan, Fu Yu, Quan Bi, Wang Shaokun, Zhang Jihong
Pages: 2651-2654
Year: 2017 Issue: 16
Journal: Journal of Modern Oncology
Keyword: acute myeloid leukemia; childhood; immunophenotype; flow cytometry;
Abstract: Objective:To investigate the immune phenotypic characteristics of childhood acute myeloid leukemia(AML) and its clinical significance.Methods:The immune phenotype in 127 cases with childhood AML were detected by four color flow cytometry with CD45/SSC double parameters scatter plot setting as door,and then the antigentic expressions were analysed.Results:In 127 patients with childhood AML,the Myeloid antigen of CD33,CD13,CD117 showed highly expressed.The positive rates were 95.3%,90.6%,90.6%.Hematopoietic stem/progenitor cell marker antigen CD34,HLA-DR and CD38 were expressed in AML with the rates of 53.5%,71.6% and 97.6%.The lymphoid antigen was positive in 65.4%of cases,with the highest frequency of CD56(38.6%)followed by CD7(21.3%).The expression of CD34+ and HLA-DR+ patients in LymAg+ group were significantly higher than LymAg-group(P<0.05).Conclusion:The immunophenotyping played an important role in diagnosis and classification of childhood AML.
Pages: 2651-2654
Year: 2017 Issue: 16
Journal: Journal of Modern Oncology
Keyword: acute myeloid leukemia; childhood; immunophenotype; flow cytometry;
Abstract: Objective:To investigate the immune phenotypic characteristics of childhood acute myeloid leukemia(AML) and its clinical significance.Methods:The immune phenotype in 127 cases with childhood AML were detected by four color flow cytometry with CD45/SSC double parameters scatter plot setting as door,and then the antigentic expressions were analysed.Results:In 127 patients with childhood AML,the Myeloid antigen of CD33,CD13,CD117 showed highly expressed.The positive rates were 95.3%,90.6%,90.6%.Hematopoietic stem/progenitor cell marker antigen CD34,HLA-DR and CD38 were expressed in AML with the rates of 53.5%,71.6% and 97.6%.The lymphoid antigen was positive in 65.4%of cases,with the highest frequency of CD56(38.6%)followed by CD7(21.3%).The expression of CD34+ and HLA-DR+ patients in LymAg+ group were significantly higher than LymAg-group(P<0.05).Conclusion:The immunophenotyping played an important role in diagnosis and classification of childhood AML.
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