Bone marrow mesenchymal stem cell transplantation reduces ischemic brain injury in a dog model of acute cerebral infarction
Author(s): LENG Hui-Lin, CAI Long
Pages: 266-269
Year: 2017 Issue: 3
Journal: Journal of International Neurology and Neurosurgery
Keyword: bone marrow mesenchymal stem cell; cerebral infarction; cell apoptosis; cytokine;
Abstract: Objective To investigate the protective effect of bone marrow mesenchymal stem cells (BMSCs) on acute ischemic brain tissue and possible mechanisms.Methods A total of 24 hybridized adult dogs were randomized into treatment group and control group.A model of ischemia was established by digital subtraction angiography-guided middle cerebral artery occlusion using autologous blood clots.Bone marrow aspiration was performed to collect BMSCs,and then BMSCs were subcultured and labeled by 4',6-diamidino-2-phenylindole (DAPI).Craniotomy was performed at 1 week after modeling and BMSCs were transplanted via intracerebral injection at multiple sites.At 1 week after transplantation,brain diffusion-weighted imaging was performed to calculate infarct volume.The dogs were sacririced at 4 weeks after transplantation,6 dogs were randomly selected from each group to collect brain tissue,and TTC staining was performed to measure infarct volume;HE staining,Van Gieson staining,and TUNEL staining were performed for the other 6 dogs in each group to evaluate brain injury.Immunofluorescent staining was performed to measure the expression of brain-derived neurotrophic factor (BDNF),basic fibroblast growth factor (bFGF),insulin-like growth factor-1 (IGF-1),and vascular endothelial growth factor (VEGF).Results The treatment group had many DAPI-positive cells widely dispersed in infarct.The treatment group had a significantly smaller infarct volume than the control group (P ＜ 0.01).Compared with the control group,the treatment group had significant alleviation in infarct extent,cell necrosis in infarct,gliosis,and glial scar.The treatment group had a significantly lower number of apoptotic cells than the control group (P ＜0.05).Compared with the control group,the treatment group had significantly higher numbers of cells with positive expression of BDNF,bFGF,IGF-1,and VEGF.Conclusions When transplantation of BMSCs is performed after cerebral infarction,BMSCs can survive,migrate to infarct,and then protect the brain,possibly by secreting various neurotrophic factors.
Pages: 266-269
Year: 2017 Issue: 3
Journal: Journal of International Neurology and Neurosurgery
Keyword: bone marrow mesenchymal stem cell; cerebral infarction; cell apoptosis; cytokine;
Abstract: Objective To investigate the protective effect of bone marrow mesenchymal stem cells (BMSCs) on acute ischemic brain tissue and possible mechanisms.Methods A total of 24 hybridized adult dogs were randomized into treatment group and control group.A model of ischemia was established by digital subtraction angiography-guided middle cerebral artery occlusion using autologous blood clots.Bone marrow aspiration was performed to collect BMSCs,and then BMSCs were subcultured and labeled by 4',6-diamidino-2-phenylindole (DAPI).Craniotomy was performed at 1 week after modeling and BMSCs were transplanted via intracerebral injection at multiple sites.At 1 week after transplantation,brain diffusion-weighted imaging was performed to calculate infarct volume.The dogs were sacririced at 4 weeks after transplantation,6 dogs were randomly selected from each group to collect brain tissue,and TTC staining was performed to measure infarct volume;HE staining,Van Gieson staining,and TUNEL staining were performed for the other 6 dogs in each group to evaluate brain injury.Immunofluorescent staining was performed to measure the expression of brain-derived neurotrophic factor (BDNF),basic fibroblast growth factor (bFGF),insulin-like growth factor-1 (IGF-1),and vascular endothelial growth factor (VEGF).Results The treatment group had many DAPI-positive cells widely dispersed in infarct.The treatment group had a significantly smaller infarct volume than the control group (P ＜ 0.01).Compared with the control group,the treatment group had significant alleviation in infarct extent,cell necrosis in infarct,gliosis,and glial scar.The treatment group had a significantly lower number of apoptotic cells than the control group (P ＜0.05).Compared with the control group,the treatment group had significantly higher numbers of cells with positive expression of BDNF,bFGF,IGF-1,and VEGF.Conclusions When transplantation of BMSCs is performed after cerebral infarction,BMSCs can survive,migrate to infarct,and then protect the brain,possibly by secreting various neurotrophic factors.
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