Correlation between neuronal autophagy and apoptosis in mouse cortex after high-altitude hypoxia treatment
Author(s): MA Wenke, DAI Shuhui, YANG Yuefan, LUO Peng, FEI Zhou
Pages: 317-320
Year: 2017 Issue: 4
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: High-altitude hypoxia; Neurons; Autophagy; Apoptosis;
Abstract: Objective Effect of high-altitude (6000 m) hypoxia exposure for different duration on neuronal autophagy and apoptosis in mouse cortex was investigated,and the correlation between them was discussed.Methods Forty C57BL/6 mice were randomly divided into five groups:control group,and 1 d,3 d,7 d,14 d high-altitude hypoxia exposure groups.High-altitude hypoxia circumstances (6000 m) was mimicked by hypobaric oxygen chamber.Then all the experimental groups were subjected to high-altitude hypoxia treatment for different durations,whereas the control group was kept only outside the chamber.Expressions of hypoxia-inducible factor 1oα (HIF-1α) and autophagy marker proteins-Bcelin-1 and microtubule-associated protein 1 light 3-Ⅱ (LC3-Ⅱ) were detected by Western Blot.Hoechst 33342 staining was used to observe the neuronal apoptosis in mouse cortex.Results Compared with control group,the expressions of HIF-1α,Beclin-1 and LC3-Ⅱ were significantly increased after high-altitude hypoxia exposure for different time.Meanwhile,neuronal apoptosis rates in mouse cortex were also increased after high-altitude hypoxia treatment.Conclusion Activation of autophagy may lead to neuronal apoptosis in mouse cortex after high-altitude hypoxia exposure and HIF-1α may play a regulatory role in this process.
Pages: 317-320
Year: 2017 Issue: 4
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: High-altitude hypoxia; Neurons; Autophagy; Apoptosis;
Abstract: Objective Effect of high-altitude (6000 m) hypoxia exposure for different duration on neuronal autophagy and apoptosis in mouse cortex was investigated,and the correlation between them was discussed.Methods Forty C57BL/6 mice were randomly divided into five groups:control group,and 1 d,3 d,7 d,14 d high-altitude hypoxia exposure groups.High-altitude hypoxia circumstances (6000 m) was mimicked by hypobaric oxygen chamber.Then all the experimental groups were subjected to high-altitude hypoxia treatment for different durations,whereas the control group was kept only outside the chamber.Expressions of hypoxia-inducible factor 1oα (HIF-1α) and autophagy marker proteins-Bcelin-1 and microtubule-associated protein 1 light 3-Ⅱ (LC3-Ⅱ) were detected by Western Blot.Hoechst 33342 staining was used to observe the neuronal apoptosis in mouse cortex.Results Compared with control group,the expressions of HIF-1α,Beclin-1 and LC3-Ⅱ were significantly increased after high-altitude hypoxia exposure for different time.Meanwhile,neuronal apoptosis rates in mouse cortex were also increased after high-altitude hypoxia treatment.Conclusion Activation of autophagy may lead to neuronal apoptosis in mouse cortex after high-altitude hypoxia exposure and HIF-1α may play a regulatory role in this process.
Related Articles