Author(s): Ji Yuanyuan, Wang Zhidong, Chen Haiyan, Wang Baotai, Yang Zheng'an, Hui Bo, Shi Min, Zhuo Fei
Pages: 1853-1858
Year: 2017 Issue:  12
Journal: Journal of Modern Oncology
Keyword:  growth and proliferation; HepG2 cells;
Abstract: Objective:To probe the influence of IGF-Ⅱ on changes of biological phenotype and expressions of antioncogenes p16 and p53 in HepG2 cells.Methods:HepG2 cells were cultured and stimulated with IGF-Ⅱ(25,50 and 100 ng/ml) for 24,48 and 72 h,changes of biological phenotype were observed.And HepG2 cells were stimulated with IGF-Ⅱ(50 ng/ml) for 48 h,mRNA levels and protein expressions of antioncogenes p16 and p53 were examined by Real-time PCR and Western blot.Moreover,expressions of p16 and p53 were detected by immunofluorescence.Results:HepG2 cells induced by IGF-Ⅱ showed a morphological alteration.MTT,Brdu and clone-formation assay suggested that growth and proliferation of HepG2 cells were enhanced by IGF-Ⅱ,but no significant difference was found between IGF-Ⅱ groups with control group(P>0.05).Compared with the control,mRNA levels of p16 and p53 were significantly decreased by IGF-Ⅱ(P<0.05) in HepG2 cells,and protein expressions of p16 and p53 were remarkably reduced by IGF-Ⅱ(P<0.05) in HepG2 cells.Furthermore,immunofluorescent expressions of p16 and p53 could be found in the nucleus of HepG2 cells.Compared with the control,immunofluorescent expressions of p16 and p53 were distinctly declined by IGF-Ⅱ in HepG2 cells(P<0.05).Conclusion:IGF-Ⅱ might induce changes of biological phenotype and inhibit expressions of antioncogenes p16 and p53 in HepG2 cells,further promoting the development of hepatocellular carcinoma.