Research on the anti-angiogenic activity of tumor suppressor C53
Author(s): ZHANG Mengmeng, HAN Yan, LI Xinyu
Pages: 381-386
Year: 2017 Issue: 4
Journal: Oncology Progress
Keyword: tumor suppressor; tumor; angiogenesis; human umbilical vein endothelial cell;
Abstract: Objective To study the anti-angiogenic activity of C53 and its molecular mechanisms in human umbilical vein endothelial cells (HUVEC) under artificial tumor-like hypoxic environment. Method The HUVEC in study group were treated hypoxically at 2%O2 (for 12 hours, 24 hours, 36 hours, and 48 hours, respectively), while the control group was given normoxic treatment. After treatment, the HUVEC and the culture supernatant of all groups were collected re-spectively for detection of cellular C53, p38MAPK, phos-p38MAPK proteins and secreted VEGF. HUVEC from the 5 groups were cultured in collected supernatant respectively to observe the angiogenetic ability of cells. Result Compared with the control group, HUVEC after hypoxic treatment of 36 h and 48 h presented less C53 along with higher level of p38MAPK phosphorylation. Hypoxic treatments of 12 h, 24 h, 36 h and 48h could markedly promote secretion of VEGF in HUVEC (P<0.05). In contrast to the supernatant from control group, supernatant collected from hypoxic treatment groups significantly promoted the proliferation, migration and tube formation ability of HUVEC, especially after treat-ment for 36 h and 48 h (P<0.05). Conclusion Hypoxic microenvironment in tumor may inhibit C53, simultaneously alle-viating inhibition of p38MAPK pathway to enhance VEGF secretion and angiogenesis.
Pages: 381-386
Year: 2017 Issue: 4
Journal: Oncology Progress
Keyword: tumor suppressor; tumor; angiogenesis; human umbilical vein endothelial cell;
Abstract: Objective To study the anti-angiogenic activity of C53 and its molecular mechanisms in human umbilical vein endothelial cells (HUVEC) under artificial tumor-like hypoxic environment. Method The HUVEC in study group were treated hypoxically at 2%O2 (for 12 hours, 24 hours, 36 hours, and 48 hours, respectively), while the control group was given normoxic treatment. After treatment, the HUVEC and the culture supernatant of all groups were collected re-spectively for detection of cellular C53, p38MAPK, phos-p38MAPK proteins and secreted VEGF. HUVEC from the 5 groups were cultured in collected supernatant respectively to observe the angiogenetic ability of cells. Result Compared with the control group, HUVEC after hypoxic treatment of 36 h and 48 h presented less C53 along with higher level of p38MAPK phosphorylation. Hypoxic treatments of 12 h, 24 h, 36 h and 48h could markedly promote secretion of VEGF in HUVEC (P<0.05). In contrast to the supernatant from control group, supernatant collected from hypoxic treatment groups significantly promoted the proliferation, migration and tube formation ability of HUVEC, especially after treat-ment for 36 h and 48 h (P<0.05). Conclusion Hypoxic microenvironment in tumor may inhibit C53, simultaneously alle-viating inhibition of p38MAPK pathway to enhance VEGF secretion and angiogenesis.
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