Immunomodulation of activated BV2 microglia by cannabinoid 1 receptor antagonist
Author(s): Li Lin, Cheng Jie, Lou Zhiyin, Zhao Zhongxin
Pages: 122-125,163
Year: 2017 Issue: 2
Journal: Journal of Navy Medicine
Keyword: Cannabinoid 1 receptor; Microglia; Cytokines; Chemokine;
Abstract: Objective To observe the immunomodulation of activated BV2 microglia by cannabinoid 1 receptor (CBIR) antagonist.Methods IFN-γ was used to stimulate BV2 microglia,and the cell model for the simulation of EAE inflammatory environment was developed.The expression levels of the CB1R mRNA and the protein in the rest BV2 group,the activated BV2 group and the SR1 intervention group were detected respectively.The concentrations of cytokines and chemokines were monitored by ELISA method,the concentration of nitric oxide was detected by Griess reagent,and proliferation rate of cells was detected by MTT.Results The expression levels of CB1RmRNA and protein of the activated BV2 were significantly higher than those of the rest BV2 group (P ＜ 0.05).Cannabinoid 1 receptor antagonist SR1 could decrease the expression levels of CB1RmRNA and protein of the activated BV2 (P ＜ 0.05).SR1 could significantly up-regulate the expression levels of IFN-γ,IL-6 and TNF-α,increase the releasing level of NO of BV2 (P ＜ 0.05) and significantly down-regulate the expression levels of IL-4,IL-17 and MCP-1.However,it had no effect on the regulation of IL-10,IL-1β and CX3CL1,and also it had no effect on the proliferation of the IFN-γ-activated BV2 (P ＞ 0.05).Conclusion CB1R was involved in the immunoregulation mediated by microglia,CB1R played a certain role in the regulation of cytokine network balance and NO secretion.
Pages: 122-125,163
Year: 2017 Issue: 2
Journal: Journal of Navy Medicine
Keyword: Cannabinoid 1 receptor; Microglia; Cytokines; Chemokine;
Abstract: Objective To observe the immunomodulation of activated BV2 microglia by cannabinoid 1 receptor (CBIR) antagonist.Methods IFN-γ was used to stimulate BV2 microglia,and the cell model for the simulation of EAE inflammatory environment was developed.The expression levels of the CB1R mRNA and the protein in the rest BV2 group,the activated BV2 group and the SR1 intervention group were detected respectively.The concentrations of cytokines and chemokines were monitored by ELISA method,the concentration of nitric oxide was detected by Griess reagent,and proliferation rate of cells was detected by MTT.Results The expression levels of CB1RmRNA and protein of the activated BV2 were significantly higher than those of the rest BV2 group (P ＜ 0.05).Cannabinoid 1 receptor antagonist SR1 could decrease the expression levels of CB1RmRNA and protein of the activated BV2 (P ＜ 0.05).SR1 could significantly up-regulate the expression levels of IFN-γ,IL-6 and TNF-α,increase the releasing level of NO of BV2 (P ＜ 0.05) and significantly down-regulate the expression levels of IL-4,IL-17 and MCP-1.However,it had no effect on the regulation of IL-10,IL-1β and CX3CL1,and also it had no effect on the proliferation of the IFN-γ-activated BV2 (P ＞ 0.05).Conclusion CB1R was involved in the immunoregulation mediated by microglia,CB1R played a certain role in the regulation of cytokine network balance and NO secretion.
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