Effect of compound matrine combined with 5-fluorouracil on inhibiting proliferation of colon cancer via regulating CyclinD1 molecule
Author(s): LYU Chi, LIU Zhao-zhe, LIU Yu-hui, HAN Tao, ZHANG Xue-feng
Pages: 156-158
Year: 2017 Issue: 2
Journal: Clinical Journal of Medical Officer
Keyword: Colon cancer; Compound matrine; 5-fluorouracil; CyclinD1;
Abstract: Objective To explore compound matrine combined with 5-fluorouracil (5-Fu)on the proliferation and apoptosis of colon cancer cell(SW480) and the expression of CyclinD1.Methods To observe the proliferation and apoptosis of SW480 cell interfered in combined treatment group (compound matrine combined 5-Fu)and the control group (dimethyl sulfoxide combined 5-Fu)using CCK-8cell proliferation experiment and flow cytometry;the expression of CyclinD1 in SW480 cell interfered between the two groups using Western blot.Results The proliferation ability of SW480 interfered in the combined treatment group was lower than that in the control group,the proliferation status on 3 points of 48 hours,72 hours and 96 hours had statistically significant difference between the two groups(P ＜ 0.05).The apoptosis rate of SW480 cells in the combined treatment group(12.7％)was higher than that in the control group(6.18％),the CyclinD1 expression of SW480 interfered in the combined treatment group was less than that in the control group (P ＜ 0.05).Conclusion Compound matrine combined with 5-Fu can significantly inhibit the proliferation of colon cancer cell (SW480) and accelerate the apoptosis of SW480 cell.The mechanism of curative effect on colon cancer may be related to inhibiting the expression of CyclinD1.
Pages: 156-158
Year: 2017 Issue: 2
Journal: Clinical Journal of Medical Officer
Keyword: Colon cancer; Compound matrine; 5-fluorouracil; CyclinD1;
Abstract: Objective To explore compound matrine combined with 5-fluorouracil (5-Fu)on the proliferation and apoptosis of colon cancer cell(SW480) and the expression of CyclinD1.Methods To observe the proliferation and apoptosis of SW480 cell interfered in combined treatment group (compound matrine combined 5-Fu)and the control group (dimethyl sulfoxide combined 5-Fu)using CCK-8cell proliferation experiment and flow cytometry;the expression of CyclinD1 in SW480 cell interfered between the two groups using Western blot.Results The proliferation ability of SW480 interfered in the combined treatment group was lower than that in the control group,the proliferation status on 3 points of 48 hours,72 hours and 96 hours had statistically significant difference between the two groups(P ＜ 0.05).The apoptosis rate of SW480 cells in the combined treatment group(12.7％)was higher than that in the control group(6.18％),the CyclinD1 expression of SW480 interfered in the combined treatment group was less than that in the control group (P ＜ 0.05).Conclusion Compound matrine combined with 5-Fu can significantly inhibit the proliferation of colon cancer cell (SW480) and accelerate the apoptosis of SW480 cell.The mechanism of curative effect on colon cancer may be related to inhibiting the expression of CyclinD1.
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