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Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater

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Abstract

Strain 11T was isolated from water of an artificial lake accumulating industrial wastewater on the outskirts of Celje, Slovenia. Phenotypic characterisation showed strain 11T to be a Gram-stain positive, spore forming bacterium. The 16S rRNA gene sequence identified strain 11T as a member of the genus Paenibacillus, closely related to Paenibacillus alvei (96.2%). Genomic similarity with P. alvei 29T was 73.1% (gANI), 70.2% (ANIb), 86.7% (ANIm) and 21.7 ± 2.3% (GGDC). The DNA G+C content of strain 11T was determined to be 47.5%. The predominant menaquinone of strain 11T was identified as MK-7 and the major fatty acid as anteiso-C15:0. The peptidoglycan was found to contain meso-diaminopimelic acid. In contrast to its close relatives P. alvei DSM 29T, Paenibacillus apiarius DSM 5581T and Paenibacillus profundus NRIC 0885T, strain 11T was found to be able to ferment d-fructose, d-mannose and d-xylose. A draft genome of strain 11T contains a cluster of genes associated with type IV pilin synthesis usually found in clostridia, and only sporadically in other Gram-positive bacteria. Genotypic, chemotaxonomic, physiological and biochemical characteristics of strain 11T presented in this study support the creation of a novel species within the genus Paenibacillus, for which the name Paenibacillus aquistagni sp. nov. is proposed, with strain 11T (=ZIM B1027T =LMG 29561T =CCM 8679T ) as the type strain.

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Acknowledgements

This research was partially funded by the Slovenian Research Agency through programme P2-0006. The genome sequencing was conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, and supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The work performed by the BCCM/LMG Bacteria Collection was supported by the Federal Public Planning Service—Science Policy, Belgium. Stefanie Van Trappen from the BCCM/LMG Bacteria Collection is acknowledged for the FAME analysis of Paenibacillus aquistagni 11T. Analysis of respiratory quinones were carried out by the Identification Service and Dr. Brian Tindall from DSMZ.

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Authors and Affiliations

  1. Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia

    Lučka Simon, Jure Škraban & Janja Trček

  2. DOE Joint Genome Institute, Walnut Creek, CA, USA

    Nikos C. Kyrpides, Tanja Woyke & Nicole Shapiro

  3. BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium

    Ilse Cleenwerck & Peter Vandamme

  4. Department of Microbiology, University of Georgia, Athens, GA, USA

    William B. Whitman

  5. Department of Biology, Faculty of Chemistry and Chemical Engineering, University of Maribor, Koroška cesta 160, 2000, Maribor, Slovenia

    Janja Trček

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  1. Lučka Simon
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Simon, L., Škraban, J., Kyrpides, N.C. et al. Paenibacillus aquistagni sp. nov., isolated from an artificial lake accumulating industrial wastewater. Antonie van Leeuwenhoek 110, 1189–1197 (2017). https://doi.org/10.1007/s10482-017-0891-x

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