Effect and safety evaluation of 50-Hz 1-mT pulsed electromagnetic field on the immunomodulation of human umbilical cord mesenchymal stem cells
Author(s): Cao Fang, Zhang Huanting, Liu Qiang, Du Liqing, Cao Xiaocang
Pages: 113-120
Year: 2017 Issue: 2
Journal: International Journal of Radiation Medicine and Nuclear Medicine
Keyword: Mesenchymal stem cell; Radiation; Pulsed electromagnetic field; Immunomodulation;
Abstract: Objective To investigate the effect of 50-Hz,1-mT pulsed electromagnetic field (PEMF) on the immunomodulation of human umbilical cord mesenchymal stem cells(MSCs) and to evaluate the safety of PEMF as a therapeutic strategy.Methods Changes in cell viability was observed via MTT.Real-time PCR was used to detect the expression levels of cyclooxygenase 2(COX2),indoleamine 2,3-dioxygenase(IDO),IL-I0,hepatocyte growth factor(HGF),and transforming growth factor-β(TGF-β) in MSCs.To explore the safety of PEMF,a flow cytometry experiment was conducted to observe apoptosis and reactive oxygen species(ROS) level.DNA damage was detected via comet assay.Data were statistically analyzed via t-test with SPSS 19.0.P＜0.05 was considered statistically significant.Results Cell viability increased,with the most obvious increase in 4 h/d(t=3.505,P＜0.05).In interferon-γ(INF-γ)-activated MSC,the expression levels of immunomodulation factors improved after PEMF irradiation(t=2.436-3.747,all P＜0.05).Furthermore,PEMF did not induce cell apoptosis,ROS generation,and DNA damage.Conclusion 50-Hz,1-mT PEMF improves MSC immunomodulation and may not cause radiation injury.
Pages: 113-120
Year: 2017 Issue: 2
Journal: International Journal of Radiation Medicine and Nuclear Medicine
Keyword: Mesenchymal stem cell; Radiation; Pulsed electromagnetic field; Immunomodulation;
Abstract: Objective To investigate the effect of 50-Hz,1-mT pulsed electromagnetic field (PEMF) on the immunomodulation of human umbilical cord mesenchymal stem cells(MSCs) and to evaluate the safety of PEMF as a therapeutic strategy.Methods Changes in cell viability was observed via MTT.Real-time PCR was used to detect the expression levels of cyclooxygenase 2(COX2),indoleamine 2,3-dioxygenase(IDO),IL-I0,hepatocyte growth factor(HGF),and transforming growth factor-β(TGF-β) in MSCs.To explore the safety of PEMF,a flow cytometry experiment was conducted to observe apoptosis and reactive oxygen species(ROS) level.DNA damage was detected via comet assay.Data were statistically analyzed via t-test with SPSS 19.0.P＜0.05 was considered statistically significant.Results Cell viability increased,with the most obvious increase in 4 h/d(t=3.505,P＜0.05).In interferon-γ(INF-γ)-activated MSC,the expression levels of immunomodulation factors improved after PEMF irradiation(t=2.436-3.747,all P＜0.05).Furthermore,PEMF did not induce cell apoptosis,ROS generation,and DNA damage.Conclusion 50-Hz,1-mT PEMF improves MSC immunomodulation and may not cause radiation injury.
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