Effects of Tanshinone ⅡA on HIF-1α and VEGF expression in brain tissue of intracerebral hemorrhage rats
Author(s): ZHAO Hongling, LI Lei, MIAO Cheng, YANG Hui, CHANG Wenguang
Pages: 130-132
Year: 2017 Issue: 2
Journal: Journal of Apoplexy and Nervous Diseases
Keyword: Intracerebral hemorrhage; Tanshinone ⅡA;
Abstract: Objective To investigate the improvement of Tanshinone ⅡA on intracerebral hemorrhage ( ICH) the effects on HIF-1α and VEGF expression .Methods Rats were randomly divided into 4 groups:sham,ICH,ICH treated with TanshinoneⅡA once a day and ICH treated with Tanshinone IIA twice a day .In addition to sham rats ,the rats were injected with collagenase to established ICH model ,and then were injected with 30 mg/kg of TanshinoneⅡA intraperitoneally once or twice a day.At the end of 15-day experimental period ,rats neural function of each group was scored and the water content in brain were calculated accordingly .The expression of HIF-1αand VEGF were determined by western blot and quantitative PCR,respectively.Results Compared with ICH group,the neural function was significantly improved and the water content in brain was decreased after TanshinoneⅡA administration.Moreover,Tanshinone IIA treatment resulted in a markedly in-crease of HIF-1αand VEGF expression when compared with ICH rats .Conclusion TanshinoneⅡA treatment can improve the neural function in brain tissue of ICH rats ,which may be related with the regulation of HIF-1αand VEGF expression .
Pages: 130-132
Year: 2017 Issue: 2
Journal: Journal of Apoplexy and Nervous Diseases
Keyword: Intracerebral hemorrhage; Tanshinone ⅡA;
Abstract: Objective To investigate the improvement of Tanshinone ⅡA on intracerebral hemorrhage ( ICH) the effects on HIF-1α and VEGF expression .Methods Rats were randomly divided into 4 groups:sham,ICH,ICH treated with TanshinoneⅡA once a day and ICH treated with Tanshinone IIA twice a day .In addition to sham rats ,the rats were injected with collagenase to established ICH model ,and then were injected with 30 mg/kg of TanshinoneⅡA intraperitoneally once or twice a day.At the end of 15-day experimental period ,rats neural function of each group was scored and the water content in brain were calculated accordingly .The expression of HIF-1αand VEGF were determined by western blot and quantitative PCR,respectively.Results Compared with ICH group,the neural function was significantly improved and the water content in brain was decreased after TanshinoneⅡA administration.Moreover,Tanshinone IIA treatment resulted in a markedly in-crease of HIF-1αand VEGF expression when compared with ICH rats .Conclusion TanshinoneⅡA treatment can improve the neural function in brain tissue of ICH rats ,which may be related with the regulation of HIF-1αand VEGF expression .
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