Cyclopamine inhibits the growth of human glioblastoma A172 cells in vivo and in vitro
Author(s): XUE Lixin, YANG Chunfu
Pages: 20-24
Year: 2017 Issue: 1
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: Human glioblastoma; Hedgehog signaling pathway; Cyclopamine; Proliferation; Apoptosis; Nude mice;
Abstract: Objective The study airs to investigate Hedgehog signaling pathway specific blocker (cyclopamine) effects human glioblastoma A172 cells growth in vivo and in vitro.Methods The proliferation of A172 cells was detected with Methylcyclopentadienyl Manganese Tricarbonyl (MTT) method.Cells apoptotic rate was analyzed with the flow cytometry assay.The expressions of the tumor-related genes and proteins in A172 cells before and after cyclopamine treatment was detected with reverse transcriotion-polymerase chain reaction (RT-PCR) and Western Blot.Cyclopamine affected A172 tumor growth in nude mice.Results Cyclopamine inhibited the growth of A172 cells in a time-and dose-dependent manner.After cyclopamine treatment with different concentrations for 48 h,the apoptotic rates of A172 cells were increased in a dose-dependent manner,which were significantly higher than those of the control group (P ＜ 0.01).Furthermore,cyclopamine down-regulated the mRNA and protein levels of SMO and GLI1 in A172 cells.Cyclopamine inhibited A172 tumor growth in nude mice.Conclusion Blocking hedgehog signaling pathway inhibits human glioblastoma A172 cells growth in vivo and in vitro.
Pages: 20-24
Year: 2017 Issue: 1
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: Human glioblastoma; Hedgehog signaling pathway; Cyclopamine; Proliferation; Apoptosis; Nude mice;
Abstract: Objective The study airs to investigate Hedgehog signaling pathway specific blocker (cyclopamine) effects human glioblastoma A172 cells growth in vivo and in vitro.Methods The proliferation of A172 cells was detected with Methylcyclopentadienyl Manganese Tricarbonyl (MTT) method.Cells apoptotic rate was analyzed with the flow cytometry assay.The expressions of the tumor-related genes and proteins in A172 cells before and after cyclopamine treatment was detected with reverse transcriotion-polymerase chain reaction (RT-PCR) and Western Blot.Cyclopamine affected A172 tumor growth in nude mice.Results Cyclopamine inhibited the growth of A172 cells in a time-and dose-dependent manner.After cyclopamine treatment with different concentrations for 48 h,the apoptotic rates of A172 cells were increased in a dose-dependent manner,which were significantly higher than those of the control group (P ＜ 0.01).Furthermore,cyclopamine down-regulated the mRNA and protein levels of SMO and GLI1 in A172 cells.Cyclopamine inhibited A172 tumor growth in nude mice.Conclusion Blocking hedgehog signaling pathway inhibits human glioblastoma A172 cells growth in vivo and in vitro.
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