Abstract
The present study aimed at comparing the responses of myogenic regulatory factors and signaling pathways involved in muscle protein synthesis after a resistance training session performed in either the fasted or fed state. According to a randomized crossover study design, six young male subjects participated in two experimental sessions separated by 3 weeks. In each session, they performed a standardized resistance training. After the sessions, they received during a 4-h recovery period 6 ml/kg b.w. h of a solution containing carbohydrates (50 g/l), protein hydrolysate (33 g/l), and leucine (16.6 g/l). On one occasion, the resistance exercise session was performed after the intake of a carbohydrate-rich breakfast (B), whereas in the other session they remained fasted (F). Needle biopsies from m. vastus lateralis were obtained before (Rest), and 1 h (+1h) and 4 h (+4h) after exercise. Myogenin, MRF4, and MyoD1 mRNA contents were determined by RT-PCR. Phosphorylation of PKB (protein kinase B), GSK3, p70s6k (p70 ribosomal S6 kinase), eIF2B, eEF2 (eukaryotic elongation factor 2), ERK1/2, and p38 was measured via western blotting. Compared with F, the pre-exercise phosphorylation states of PKB and p70s6k were higher in B, whereas those of eIF2B and eEF2 were lower. During recovery, the phosphorylation state of p70s6k was lower in B than in F (p = 0.02). There were no differences in basal mRNA contents between B and F. However, compared with F at +1h, MyoD1 and MRF4 mRNA contents were lower in B (p < 0.05). Our results indicate that prior fasting may stimulate the intramyocellular anabolic response to ingestion of a carbohydrate/protein/leucine mixture following a heavy resistance training session.
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Acknowledgments
This study was supported by grants from the Onderzoeksraad K.U.-Leuven (grant # OT04/45), the Fonds voor Wetenschappelijk Onderzoek Vlaanderen (grant # G.0233.05) and the Fonds de la Recherche Scientifique Médicale (grant # 3.4574.03). Hyprol 4107 was kindly provided by Kerry Bio-Science, Utrecht, the Netherlands. Louise Deldicque is supported by the «Fonds National de la Recherche Scientifique (FNRS)».
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Communicated by Håkan Westerblad.
L. Deldicque and K. De Bock contributed equally to this work.
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Deldicque, L., De Bock, K., Maris, M. et al. Increased p70s6k phosphorylation during intake of a protein–carbohydrate drink following resistance exercise in the fasted state. Eur J Appl Physiol 108, 791–800 (2010). https://doi.org/10.1007/s00421-009-1289-x
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DOI: https://doi.org/10.1007/s00421-009-1289-x