Effect of RNA Interference Silencing ANTXR1 Gene on Proliferation and Apoptosis of Esophageal Cancer ECA109 Cells
Author(s): REN Yong, HE Wei, QI Man-li, CHEN Xin-wei, LIANG Li-wei, FENG Jun-ming, Department of Pathology, Wuhan General Hospital of Chinese People’s Liberation Army
Pages: 761-765
Year: 2016 Issue: 12
Journal: Military Medical Journal of South China
Keyword: Anthrax toxin receptor 1; Small interference RNA; Esophageal squamous cell carcinoma; Cell proliferation; Cell apoptosis;
Abstract: Objective To explore the effects of expression down-regulation of anthrax toxin receptor 1(ANTXR1)gene induced by RNA interference on the proliferation and apoptosis of esophageal squamous cell carcinoma(ESCC)ECA109 cells.Methods Small interfering RNA(siRNA)against the ANTXR1 gene was transfected into ECA109 cells using LipofectamineTM2000.After transfection,ANTXR1 expression was detected by quantitative real-time polymerase chain reaction(RT-PCR)and Western blot,respectively;cell proliferation was determined using cell counting kit-8(CCK-8)and plate clone formation assay.The distribution of cell cycle and apoptosis were assessed by flow cytometry.Results ANTXR1 expression was significantly down-regulated after transfection with ANTXR1 siRNA(P<0.01).Compared with negative control group,the proliferation rate of ECA109 cells was significantly restrained after transfection with ANTXR1 siRNA for 3,4 and 5 days by CCK-8 assay,and the number of clones was significantly decreased by plate clone formation assay.The percentage of cells at G1 phase(65.90%±6.87%)in RNAi group was increased comparing with negative control group(40.17%±4.73%)(P<0.05),and the early apoptosis rate of ECA109 cells transfected with ANTXR1 siRNA in 48 hours was significantly increased [(22.61% ±3.49%)vs.(8.12% ±1.35%),P<0.01)].Conclusion Silencing ANTXR1 gene can inhibit the proliferation and promote the apoptosis of ECA109 cells.It shows that ANTXR1 plays a dominant role in the development of ESCC and it may be a new therapeutic target of ESCC.
Pages: 761-765
Year: 2016 Issue: 12
Journal: Military Medical Journal of South China
Keyword: Anthrax toxin receptor 1; Small interference RNA; Esophageal squamous cell carcinoma; Cell proliferation; Cell apoptosis;
Abstract: Objective To explore the effects of expression down-regulation of anthrax toxin receptor 1(ANTXR1)gene induced by RNA interference on the proliferation and apoptosis of esophageal squamous cell carcinoma(ESCC)ECA109 cells.Methods Small interfering RNA(siRNA)against the ANTXR1 gene was transfected into ECA109 cells using LipofectamineTM2000.After transfection,ANTXR1 expression was detected by quantitative real-time polymerase chain reaction(RT-PCR)and Western blot,respectively;cell proliferation was determined using cell counting kit-8(CCK-8)and plate clone formation assay.The distribution of cell cycle and apoptosis were assessed by flow cytometry.Results ANTXR1 expression was significantly down-regulated after transfection with ANTXR1 siRNA(P<0.01).Compared with negative control group,the proliferation rate of ECA109 cells was significantly restrained after transfection with ANTXR1 siRNA for 3,4 and 5 days by CCK-8 assay,and the number of clones was significantly decreased by plate clone formation assay.The percentage of cells at G1 phase(65.90%±6.87%)in RNAi group was increased comparing with negative control group(40.17%±4.73%)(P<0.05),and the early apoptosis rate of ECA109 cells transfected with ANTXR1 siRNA in 48 hours was significantly increased [(22.61% ±3.49%)vs.(8.12% ±1.35%),P<0.01)].Conclusion Silencing ANTXR1 gene can inhibit the proliferation and promote the apoptosis of ECA109 cells.It shows that ANTXR1 plays a dominant role in the development of ESCC and it may be a new therapeutic target of ESCC.
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