Determination of Related Substance in Betacyclodextrin by Ion Chromatography with Integral Pulse Amperometric Detection
Author(s): Wang Miao, Jin Guiying, Wang Caimei
Pages: 27-31
Year: 2017 Issue: 4
Journal: China Pharmaceuticals
Keyword: betacyclodextrin; ion chromatography; integral impulse amperometric detection; related substance;
Abstract: Objective To establish a method of ion chromatography with integral pulse amperometric detection for the determination of related substance in betacyclodextrin. Methods Dionex ICS-500 and CarPac PA200 column were used. The mixtures of pure water, 250 mmol/L NaOH solution and 500 mmol/L NaAC solution in different ratio were used as mobile phase in the gradient elution. In the amperometric detection,gold electrode was used as working electrode,Ag/AgCl electrode was used as reference electrode. Results The peaks of different impurities were separated completely. The calibration curve of each solvent of each impurity showed a good liner relationship with good precision and recovery rate. Conclusion The method showed good sensitivity,precision and reproducibility,which can be used to determine the related substance in betacyclodextrin.
Pages: 27-31
Year: 2017 Issue: 4
Journal: China Pharmaceuticals
Keyword: betacyclodextrin; ion chromatography; integral impulse amperometric detection; related substance;
Abstract: Objective To establish a method of ion chromatography with integral pulse amperometric detection for the determination of related substance in betacyclodextrin. Methods Dionex ICS-500 and CarPac PA200 column were used. The mixtures of pure water, 250 mmol/L NaOH solution and 500 mmol/L NaAC solution in different ratio were used as mobile phase in the gradient elution. In the amperometric detection,gold electrode was used as working electrode,Ag/AgCl electrode was used as reference electrode. Results The peaks of different impurities were separated completely. The calibration curve of each solvent of each impurity showed a good liner relationship with good precision and recovery rate. Conclusion The method showed good sensitivity,precision and reproducibility,which can be used to determine the related substance in betacyclodextrin.
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