Knockdown of Survivin expression inhibits glioma cells invasion induced by bevacizumab treatment in glioma cells
Author(s): WANG Peng, ZHANG Jianning, CHEN Jinhui, YU Xin, LIU Rui, SUN Yanjie
Pages: 11-14
Year: 2017 Issue: 1
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: Glioma; Bevacizumab; Survivin; Invasion;
Abstract: Objective The study aims to investigate the effect of Survivin on glioma cells invasion after treated by bevacizumab.Methods Survivin shRNA plasmids were transfected in to U251 cells.The cells proliferation,migration,and invasion were determined by methyl thiazolyl tetrazolium (MTT) assay,wound healing,and transwell assay,respectively.Results The migration and invasion of U251 cells were increased by bevacizumab.Knockdown of Survivin by RNAi inhibited bevacizumab-induced cell invasion.Furthermore,the combined administration of bevacizumab and knockdown of Survivin significantly suppressed the proliferation of glioma cells compared to bevacizumab treatment or knockdown of Survivin alone.Conclusion Down-regulation of Survivin could inhibit glioma cells invasion induced by bevacizumab treatment,therefore Survivin might be a potential target to ameliorate the therapeutic efficiency of bevacizumab.
Pages: 11-14
Year: 2017 Issue: 1
Journal: Chinese Journal of Neurosurgical Disease Research
Keyword: Glioma; Bevacizumab; Survivin; Invasion;
Abstract: Objective The study aims to investigate the effect of Survivin on glioma cells invasion after treated by bevacizumab.Methods Survivin shRNA plasmids were transfected in to U251 cells.The cells proliferation,migration,and invasion were determined by methyl thiazolyl tetrazolium (MTT) assay,wound healing,and transwell assay,respectively.Results The migration and invasion of U251 cells were increased by bevacizumab.Knockdown of Survivin by RNAi inhibited bevacizumab-induced cell invasion.Furthermore,the combined administration of bevacizumab and knockdown of Survivin significantly suppressed the proliferation of glioma cells compared to bevacizumab treatment or knockdown of Survivin alone.Conclusion Down-regulation of Survivin could inhibit glioma cells invasion induced by bevacizumab treatment,therefore Survivin might be a potential target to ameliorate the therapeutic efficiency of bevacizumab.
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