Expression of endogenous hydrogen sulfide signal during rabbit mandibular distraction osteogenesis
Author(s): JIANG Xiao-wen, ZHANG Yi, LU Kun, ZHANG Hao, CHEN Yan-ze
Pages: 262-265
Year: 2017 Issue: 5
Journal: Beijing Journal of Stomatology
Keyword: Mandible; Distraction osteogenesis; Hydrogen sulfide; Rabbit;
Abstract: Objective To investigate the endogenous hydrogen sulfide (H2S) signal expression during distraction osteogenesis (DO) in a rabbit model. Methods A total of 34 male New Zealand white rabbits were included and underwent right mandibular osteodistraction. Five days after operation,the animals were randomly divided into group A(1mm twice a day,5 days) and group B (0.5mm twice a day,10 days). During the DO, the vein plasma was collected at five time points to determine the concentration of H2S. Four and eight weeks after completion of distraction, the animals were sacrificed and the lengthened tissue was harvested for cystathionine-γ-lyase(CSE) evaluation. The osteogenesis effect was also evaluated by CT and dual-energy x-ray absorptiometry (DXA). Results The endogenous H2S signal was detected during the whole process of DO. However,under the rapid distracted protocol (group A), the H2S signal was obviously decreased,which resulted in poor osteogenesis. Conclusion The endogenous H2S signal system may play a role during DO. The exogenous H2S might be considered as a new protocol to promote osteogensis during DO.
Pages: 262-265
Year: 2017 Issue: 5
Journal: Beijing Journal of Stomatology
Keyword: Mandible; Distraction osteogenesis; Hydrogen sulfide; Rabbit;
Abstract: Objective To investigate the endogenous hydrogen sulfide (H2S) signal expression during distraction osteogenesis (DO) in a rabbit model. Methods A total of 34 male New Zealand white rabbits were included and underwent right mandibular osteodistraction. Five days after operation,the animals were randomly divided into group A(1mm twice a day,5 days) and group B (0.5mm twice a day,10 days). During the DO, the vein plasma was collected at five time points to determine the concentration of H2S. Four and eight weeks after completion of distraction, the animals were sacrificed and the lengthened tissue was harvested for cystathionine-γ-lyase(CSE) evaluation. The osteogenesis effect was also evaluated by CT and dual-energy x-ray absorptiometry (DXA). Results The endogenous H2S signal was detected during the whole process of DO. However,under the rapid distracted protocol (group A), the H2S signal was obviously decreased,which resulted in poor osteogenesis. Conclusion The endogenous H2S signal system may play a role during DO. The exogenous H2S might be considered as a new protocol to promote osteogensis during DO.
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