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8-O-acetyl-SM attenuates chronic inflammatory pain via inhibition of JNK phosphorylation in spinal dorsal horn of rats
Pages: 317-
322
Year: 2017
Issue:
3
Journal: Chinese Journal of Neuroanatomy
Abstract: Objective:To explore effects of 8-O-acetyl-SM (8-OaS) on complete Freund's adjuvant (CFA)-induced nociceptive behavior and expressions of phosphorylated c-Jun N-terminal kinase (pJNK) of astrocytes in the spinal dorsal horn of rats.Methods:Chronic inflammatory pain was established by intraplanter injection of complete Freund's adjuvant (CFA).8-OaS was injected intraperitoneally (i.p.).yon Frey filaments were used to investigate the 50% mechanical allodynia of the planta of rats.Immunofluorescent histochemistry was applied to observe the expression of glial fibrillary acidic protein (GFAP) and pJNK.Western Blot was performed to qualitatively analyze the expression levels of GFAP,JNK and pJNK.Results:(1) Behavioral results showed that the mechanical withdrawl threshold was obviously and significantly decreased in CFA group compared to control group (P < 0.01),and i.p.treatment of 8-OaS can greatly attenuate CFA-induced mechanical allodynia (P < 0.05).(2) Immunofluorescent histochemical staining showed that the expression of GFAP was significantly increased in spinal dorsal horn of CFA rats.Moreover,it was observed that pJNK was almost entirely expressed in astrocytes.(3) Western Blot data revealed that the expression levels of GFAP and pJNK were significantly increased in CFA rats,and i.p.injection of 8-OaS could obviously inhibit the activation of astrocytes and pJNK (P < 0.01).Conclusion:i.p.administration of 8-OaS can significantly attenuate CFA-induced mechanical allodynia.The underlying mechanism of the potential analgesia effect of 8-OaS iscontributedto the suppression of phosphorylation of pJNK and the activation of astrocytes.
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