Pages: 2361-2365
Year: 2017 Issue:  15
Journal: Journal of Modern Oncology
Abstract: Objective:To explore the effect of lncRNA SNHG20 on the chemoresistance of colorectal cancer and its underlying mechanism.Methods:5-fluorouracil resistance colorectal cell line LoVo/5-FU was constructed to evaluate the expression of lncRNA SNHG20 in parental LoVo and LoVo/5-FU cells.Gain of function and loss of function assays were further used to investigate the effect of lncRNA SNHG20 on the 5-fluorouracil resistance of LoVo cells.Results:lncRNA SNHG20 was highly up-regulated in LoVo/5-FU cells in comparison with parental LoVo cells (P<0.001).Furthermore,overexpression of lncRNA SNHG20 significantly reduced the sensitivity to 5-FU in LoVo cells,whereas silencing lncRNA SNHG20 increased the sensitivity to 5-FU in LoVo/5-FU cells (P<0.001).Clone formation assay revealed that up-regulation of lncRNA SNHG20 obviously promotes cellular clonality in LoVo cells.On the contrary,knocked down of lncRNA SNHG20 visibly reduced colony forming ability in LoVo/5-FU cells (P<0.001).In addition,Western blot indicated that lncRNA SNHG20 reduced the expression of PTEN,whereas up-regulated the expression of p-AKT and p-PI3K in LoVo cells.Consistently,knocked down of lncRNA SNHG20 in LoVo/5-FU cells had the opposite effects on the expression of PTEN,p-AKT and p-PI3K.Conclusion:lncRNA SNHG20 promotes resistance to 5-FU in colorectal cancer via PTEN/PI3K/AKT signal pathway.