The purpose of this is to verify that the assay is detecting peptides based on their specific presence in the autosampler vial. We want to ensure that the machine only captures the oyster transitions we set out to measure. The oyster peptide detection should decrease as the concentration in the autosampler vial decreases, and vice versa.

to clarify you need to add brief explanation for every major step in the protocol.

Ohhh, will do!

Protocol updated accordingly.