Pages: 8-10
Year: 2017 Issue:  6
Journal: The Chinese Journal of Human Sexuality
Abstract: Objectives:To study the expression of miR-21 and programmed death factor-4 (PDCD4) mRNA in prostate cancer tissue.Methods:26 prostate cancer patients in our hospital from October 2013 to April 2016 were selected.The prostate carcinoma tissue and normal adjacent tissues of the 26 patients were taken and detected by fluorescence quantitative PCR,to determine the expression of miR-21 and PDCD4 mRNA specimens.The expression of PDCD 4 protein in prostate cancer and adjacent tissues was detected by Western blot detection and the correlation between miR-21 and the expression of PDCD4 was analyzed.Results:The expression level of miR -21 in prostate caneer tissue was higher than that in adjacent tissue (P ＜ 0.05),while the expression level of PDCD4 mRNA was significantly lower than that of adjacent tissues (P ＜ 0.05).The expression of PDCD4 protein in carcinoma gray value ratio was significantly higher than that of prostate cancer tissue (P ＜ 0.05).There was a significant negative cor,elation between miR-21 and PDCD4 mRNA expression in prostate cancer tissue (P ＜ 0.05),and the expression level was negatively correlated with PDCD4 (P ＜ 0.05).Conclusion:The miR-21 may be involved in the pathogenesis of prostate cancer by down regulating the expression level of PDCD4,which can lead to the occurrence and development of prostate cancer.