Inhibitory Effect of Schaftoside on C57BL/6 Mice with Cholesterol Gallstone Formation Induced by High-fat Diet
Author(s): LIU Meijing, LIU Changhui, HUANG Xiaotao, MI Suiqing, Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine
Pages: 497-502
Year: 2016 Issue: 4
Journal: Traditional Chinese Drug Research & Clinical Pharmacology
Keyword: Schaftoside; Cholesterol gallstone; inhibitory effect; cholesterol saturation index(CSI);
Abstract: Objective To study the inhibitory effect of schaftoside on cholesterol gallstone formation in experimental C57BL/6 mice induced by high-fat diet. Methods A total of 72 C57BL/6J mice were randomly divided into six groups,namely normal group,model group,ursodeoxycholic acid（UDCA）group(60 mg·kg-1),and low-,middle-,highdose schaftoside groups(in the dosage of 20,40,80 mg·kg-1 respectively),12 mice in each group. The mice were intragastrically administered for 8 weeks. Except for the normal group,the mice of other groups were fed with high-fat diet. Body weight and food intake were recorded every 7 days. We observed the stone formation with naked eyes and microscope, detected the levels of serum total cholesterol（TC）, high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）,total bile acid（TBA）,triglyceride（TG）,examined bile TC,TBA,phospholipid（PL）,calculated the cholesterol saturation index（CSI）,and observed the liver pathological changes under light microscope. Results Compared with the model group,high-dose schaftoside group could significantly lower the rate of stone formation（33.3 %）,increase serum HDL-C level and decrease TG level,reduce TC and PL level in gallbladder bile and increase TBA level, thus reducing the CSI. Compared with the model group, middle- and high-dose schaftoside groups relieved hepatic fatty degeneration in mice. Conclusion It is indicated that schaftoside can inhibit C57BL/6 mice cholesterol gallstone formation induced by high-fat diet,and the mechanism may be related with decreasing gallbladder bile CSI,increasing serum HDL-C level,and reduce liver damage resulting from hepatic lipid disorders.
Pages: 497-502
Year: 2016 Issue: 4
Journal: Traditional Chinese Drug Research & Clinical Pharmacology
Keyword: Schaftoside; Cholesterol gallstone; inhibitory effect; cholesterol saturation index(CSI);
Abstract: Objective To study the inhibitory effect of schaftoside on cholesterol gallstone formation in experimental C57BL/6 mice induced by high-fat diet. Methods A total of 72 C57BL/6J mice were randomly divided into six groups,namely normal group,model group,ursodeoxycholic acid（UDCA）group(60 mg·kg-1),and low-,middle-,highdose schaftoside groups(in the dosage of 20,40,80 mg·kg-1 respectively),12 mice in each group. The mice were intragastrically administered for 8 weeks. Except for the normal group,the mice of other groups were fed with high-fat diet. Body weight and food intake were recorded every 7 days. We observed the stone formation with naked eyes and microscope, detected the levels of serum total cholesterol（TC）, high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C）,total bile acid（TBA）,triglyceride（TG）,examined bile TC,TBA,phospholipid（PL）,calculated the cholesterol saturation index（CSI）,and observed the liver pathological changes under light microscope. Results Compared with the model group,high-dose schaftoside group could significantly lower the rate of stone formation（33.3 %）,increase serum HDL-C level and decrease TG level,reduce TC and PL level in gallbladder bile and increase TBA level, thus reducing the CSI. Compared with the model group, middle- and high-dose schaftoside groups relieved hepatic fatty degeneration in mice. Conclusion It is indicated that schaftoside can inhibit C57BL/6 mice cholesterol gallstone formation induced by high-fat diet,and the mechanism may be related with decreasing gallbladder bile CSI,increasing serum HDL-C level,and reduce liver damage resulting from hepatic lipid disorders.
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