Roles of endoplasmic reticulum stress and miR-375 in apoptosis of pancreatic β cells induced by hyperglycemia and hyperlipidemia
Author(s): JI Hui-zhen, YANG Jing, XING Xiao-ping, LI Yu-xiu, PAN Hui, SUN Qi, The First Clinical Medical College, Shanxi Medical University
Pages: 2065-2067+2117
Year: 2016 Issue: 14
Journal: Chinese Journal of Health Laboratory Technology
Keyword: Endoplasmic reticulum stress; miR-375; Pancreatic β cell; Apoptosis;
Abstract: Objective To investigate the roles of endoplasmic reticulum stress and miR- 375 in the apoptosis and multiplication of RINm5 F cells under different concentrations of glucose and fat. Methods RINm5 F cells were cultured in vitro under different concentrations of glucose and palmitate acid. The Cell Counting Kit- 8 was used to measure the proliferative activity of cells. Quantitative real- time PCR( qt- PCR) was performed to measure endoplasmic reticulum stress related genes XBP- 1,CHOP and miR- 375 expression,and Western blot was used to measure the expression levels of CHOP protein and caspase- 3protein. Results Under the condition of the same concentration of glucose,plamitate acid could promote the expression of XBP- 1 mRNA,CHOP,mRNA,miR- 375( P < 0. 05),and decrease the proliferation activity of pancreatic islet beta cells( P < 0. 05); Western blot detection showed that CHOP and caspase- 3 protein expression were increased. Conclusion Under the action of hyperglycemia and hyperlipidemia,the induction of endoplasmic reticulum stress and the increase of miR- 375 are related to the apoptosis of pancreatic β cells.
Pages: 2065-2067+2117
Year: 2016 Issue: 14
Journal: Chinese Journal of Health Laboratory Technology
Keyword: Endoplasmic reticulum stress; miR-375; Pancreatic β cell; Apoptosis;
Abstract: Objective To investigate the roles of endoplasmic reticulum stress and miR- 375 in the apoptosis and multiplication of RINm5 F cells under different concentrations of glucose and fat. Methods RINm5 F cells were cultured in vitro under different concentrations of glucose and palmitate acid. The Cell Counting Kit- 8 was used to measure the proliferative activity of cells. Quantitative real- time PCR( qt- PCR) was performed to measure endoplasmic reticulum stress related genes XBP- 1,CHOP and miR- 375 expression,and Western blot was used to measure the expression levels of CHOP protein and caspase- 3protein. Results Under the condition of the same concentration of glucose,plamitate acid could promote the expression of XBP- 1 mRNA,CHOP,mRNA,miR- 375( P < 0. 05),and decrease the proliferation activity of pancreatic islet beta cells( P < 0. 05); Western blot detection showed that CHOP and caspase- 3 protein expression were increased. Conclusion Under the action of hyperglycemia and hyperlipidemia,the induction of endoplasmic reticulum stress and the increase of miR- 375 are related to the apoptosis of pancreatic β cells.
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