Optimization of Enzymatic Extraction Technology of Polysaccharides from Schisandra chinensis by Central Composite Design-response Surface Methodology
Author(s): ZHANG Nannan, ZHU Zhijun, JIANG Yachao, DENG Hao, GAO Song, LI Yingli, College of Pharmacy, Henan University of Chinese Medicine
Pages: 3142-3144
Year: 2016 Issue: 22
Journal: China Pharmacy
Keyword: Schisandra chinensis polysaccharide; Enzymatic extraction technology; Central composite-response surface methodology;
Abstract: OBJECTIVE:To optimize enzymatic extraction technology of polysaccharide from Schisandra chinensis. METHODS:Using p H value of enzymatic extraction solution,the amount of enzyme,extraction temperature as response factor,S. chinensis polysaccharide as response value,on the basis of single-factor experiments,3-factor,5-level central composite experimental design was adopted for the experiment. Validation test was also conducted. RESULTS:The optimal extraction technology was as pH value of 5.7,enzyme dosage of 1.3%,extraction temperature of 53 ℃. In validation test,the extraction rate of S. chinensis polysaccharide was 14.30%(RSD=1.84%,n=6). CONCLUSIONS:The optimized extraction technology is simple,reasonable and stable,and can be used for the extraction of polysaccharide from S. chinensis.
Pages: 3142-3144
Year: 2016 Issue: 22
Journal: China Pharmacy
Keyword: Schisandra chinensis polysaccharide; Enzymatic extraction technology; Central composite-response surface methodology;
Abstract: OBJECTIVE:To optimize enzymatic extraction technology of polysaccharide from Schisandra chinensis. METHODS:Using p H value of enzymatic extraction solution,the amount of enzyme,extraction temperature as response factor,S. chinensis polysaccharide as response value,on the basis of single-factor experiments,3-factor,5-level central composite experimental design was adopted for the experiment. Validation test was also conducted. RESULTS:The optimal extraction technology was as pH value of 5.7,enzyme dosage of 1.3%,extraction temperature of 53 ℃. In validation test,the extraction rate of S. chinensis polysaccharide was 14.30%(RSD=1.84%,n=6). CONCLUSIONS:The optimized extraction technology is simple,reasonable and stable,and can be used for the extraction of polysaccharide from S. chinensis.
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