Author(s): Zhou Yan, Sun Xiaohong, Liu Yujun, Lei Huimeng, Xu Qunyuan, Department of Neurobiology, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University
Pages: 713-716
Year: 2015 Issue:  6
Journal: Chinese Journal of Neuroanatomy
Keyword:  Parkinson s disease; nerve growth factor; substantia nigra; inflammation; rat;
Abstract: Objective:To study the effect of chronic inflammation in rat brain on the expression of nerve growth factor(NGF) and the acting selectivity in the substantia nigra(SN).Methods:A single intracerebroventricular injection of lipopolysaccharide(LPS) was made to induce brain chronic inflammation model of Parkinson’ s disease(PD).At certain time points,different parts of brain were dissected and the protein was extracted and the expression of NGF in the SN and the dorsal raphe nucleus was detected with Western Blot.Results:The expression of NGF in SN was reduced significantly from 4 weeks after LPS injection compared with the control group,decreasing continuously till 24 weeks.In contrast,the expression of NGF in the dorsal raphe nucleus had no significant change during the entire time course in the LPS group compared with the control group.Conclusion:Chronic inflammation in the brain can selectively reduce the expression of NGF in the SN.It might relate to the selective reduction of dopaminergic neurons in the SN caused by the chronic inflammation in the brain.