Abstract
Physalin A (PA) is an active withanolide isolated from Physalis alkekengi var. franchetii, a traditional Chinese herbal medicine named Jindenglong, which has long been used for the treatment of sore throat, hepatitis, and tumors in China. In the present study, we firstly investigated the effects of PA on proliferation and cell cycle distribution of the human non-small cell lung cancer (NSCLC) A549 cell line, and the potential mechanisms involved. Here, PA inhibited cell growth in dose- and time-dependent manners. Treatment of A549 cells with 28.4 μM PA for 24 h resulted in approximately 50 % cell death. PA increased the amount of intracellular ROS and the proportion of cells in G2/M. G2/M arrest was attenuated by the addition of ROS scavenger NAC. ERK and P38 were triggered by PA through phosphorylation in a time-dependent manner. The phosphorylation of ERK and P38 were not attenuated by the addition of NAC, but the use of the p38 inhibitor could reduce, at least in part, PA-induced ROS and the proportion of cells in G2/M. PA induces G2/M cell cycle arrest in A549 cells involving in the p38 MAPK/ROS pathway. This study suggests that PA might be a promising therapeutic agent against NSCLC.
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Change history
02 December 2021
A Correction to this paper has been published: https://doi.org/10.1007/s11010-021-04318-8
Abbreviations
- ANOVA:
-
Analysis of variance
- DCF-DA:
-
2′,7′-Dichloro -fluorescein diacetate
- DMSO:
-
Dimethyl sulfoxide
- FBS:
-
Fetal bovine serum
- iNOS:
-
Inducible nitric oxide synthase
- LSD:
-
Least significant difference
- MAPKs:
-
Mitogen-activated protein kinases
- MPF:
-
Mitosis-promoting factor
- MTT:
-
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- NAC:
-
N-Acetylcysteine
- NO:
-
Nitric oxide
- NSCLC:
-
Non-small cell lung cancer
- PA:
-
Physalin A
- PI:
-
Propidium iodide
- ROS:
-
Reactive oxygen species
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Acknowledgments
This work was supported by National Natural Science Foundation of China (No. 21472138), the China Postdoctoral Science Foundation (No. 2013M541192), and the China Postdoctoral Science Special Foundation (No. 2014T70224).
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Kang, N., Jian, Jf., Cao, Sj. et al. Physalin A induces G2/M phase cell cycle arrest in human non-small cell lung cancer cells: involvement of the p38 MAPK/ROS pathway. Mol Cell Biochem 415, 145–155 (2016). https://doi.org/10.1007/s11010-016-2686-1
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DOI: https://doi.org/10.1007/s11010-016-2686-1