Author(s): Gan Na, Yin Fei, Peng Jing, Wu Liwen, He Fang, Chen Chen, Department of Clinical Medicine, Guangxi Medical College, Department of Pediatrics, Xiangya Hospital, Central South University
Pages: 783-787
Year: 2015 Issue:  6
Journal: Chinese Journal of Neuroanatomy
Keyword:  astrocyte; inflammation; MRP8; IL-1β; cellular culture;
Abstract: Objective:To study the pathway of IL-1β serection stimulated by MRP8 in astrocyte(AS).Methods:MRP8were used to stimulate AS in vitro,lipofection transfectional shRNA were used to inhibit TLR4,PDTC were used to inhibit NF-κB.Western Blot,EMSA,ELISA and fluorescent double labelling were used to dectect the expressions of TLR4,NFkB and IL-1β,and analyze the relationship between these cytokines.Results:MRP8 increased TLR4,NF-κB and IL-1βexpressions in astrocyte,while NF-κB transferred into the nucleus,and IL-1β expression in cell supernatant also increased.TLR4 inhibition decreased the NF-κB and IL-1β expressions and NF-κB transferred into the nucleus induced by MRP8.NF-κB inhibition only decreased IL-1β expressions induced by MRP8.Conclusion:MRP8 stimulated IL-1βserection in cultured astrocyte via TLR4/NF-κB pathway.