Expression and significance of spinal microglia and IL-1β in cancer-induced bone pain
Author(s): JIANG Jing-jing, YAO Peng, WU Xiu-ying, ZHANG Jin, Departments of Anesthesiology, Shengjing Hospital of China Medical University
Pages: 44-47
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Bone cancer pain; Spinal cord; Microglia; Hyperalgesia;
Abstract: Objective To observe the expression of microglia and IL-1β in cancer-induced bone pain(CIBP). Methods Female SD rats were randomly divided into two groups(n=48 each). Sham group:sham operation group;CIBP group:walker256 mammary gland carcinoma cell was inoculated into the rat tibia. On the pre-inoculation and day 6,14 and 21,the lumbar 4-6 spinal cord were removed for detecting the expressions of IL-1β,Immunohistochemistry was used to identify the distribution and location of microglia marker(OX42). Results Compared with sham group,the mean optical density of OX-42 was significantly increased at day 6 after inoculation(P <0.01). The inoculation of walker 256 tumor cell could significantly increase the expression of OX-42 in ipsilateral spinal cord dorsal,microglia change their morphology from having a small soma to having a hypertrophic soma.Compared with sham group,the expression of IL-1β was significantly increased from day 6 to day 21 after tumor cell inoculation in group CIBP(P <0.01). Conclusion Bone cancer induced spinal microglia activation,which promoted the release of IL-1β。
Pages: 44-47
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Bone cancer pain; Spinal cord; Microglia; Hyperalgesia;
Abstract: Objective To observe the expression of microglia and IL-1β in cancer-induced bone pain(CIBP). Methods Female SD rats were randomly divided into two groups(n=48 each). Sham group:sham operation group;CIBP group:walker256 mammary gland carcinoma cell was inoculated into the rat tibia. On the pre-inoculation and day 6,14 and 21,the lumbar 4-6 spinal cord were removed for detecting the expressions of IL-1β,Immunohistochemistry was used to identify the distribution and location of microglia marker(OX42). Results Compared with sham group,the mean optical density of OX-42 was significantly increased at day 6 after inoculation(P <0.01). The inoculation of walker 256 tumor cell could significantly increase the expression of OX-42 in ipsilateral spinal cord dorsal,microglia change their morphology from having a small soma to having a hypertrophic soma.Compared with sham group,the expression of IL-1β was significantly increased from day 6 to day 21 after tumor cell inoculation in group CIBP(P <0.01). Conclusion Bone cancer induced spinal microglia activation,which promoted the release of IL-1β。
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