Determination of Paraquat in Plasma by Reversed Phase Ion-pair HPLC
Author(s): ZHANG Hong-wen, YIN Zhi-yang, LIU Qiang-hui, CAO Yang, WANG Yu-qing, WANG Yong-qing, OU Ning, MENG Ling, ZHANG Jing-song, Department of Pharmacy, the First Affiliated Hospital of Nanjing Medical University, Department of Emergency Medicine, the First Affiliated Hospital of Nanjing Medical University, College of Pharmacy, Nanjing Medical University
Pages: 558-561
Year: 2015 Issue: 6
Journal: Jiangsu Pharmacertical and Clinical Research
Keyword: Paraquat; Ion-pair; HPLC; Plasma concentration; Intoxication;
Abstract: Objective: To establish a method for determination of paraquat in plasma by reversed phase ion-pair HPLC. Methods: The Discovery C18 column was used for separation and the mobile phase consisted of a mixture of acetonitril-sodium heptanesulfonate solution（12 ∶88, v ∶v）. The plasma proteins were precipitated by 10% trichloroacetic acid. The flow-rate was 1.0 m L ·min-1, and the detection wavelength was 258 nm. Results: The detection limit of the method was 0.1 μg·m L-1. The linear calibration curves were obtained in concentration range of 0.1-10.0 μg·m L-1. The intra- and inter-day RSD were3.77% and 10.60%, respectively. The average recovery was above 89.31%. Conclusion: The method is simple and rapid, which can be used for determination of paraquat in human plasma.
Pages: 558-561
Year: 2015 Issue: 6
Journal: Jiangsu Pharmacertical and Clinical Research
Keyword: Paraquat; Ion-pair; HPLC; Plasma concentration; Intoxication;
Abstract: Objective: To establish a method for determination of paraquat in plasma by reversed phase ion-pair HPLC. Methods: The Discovery C18 column was used for separation and the mobile phase consisted of a mixture of acetonitril-sodium heptanesulfonate solution（12 ∶88, v ∶v）. The plasma proteins were precipitated by 10% trichloroacetic acid. The flow-rate was 1.0 m L ·min-1, and the detection wavelength was 258 nm. Results: The detection limit of the method was 0.1 μg·m L-1. The linear calibration curves were obtained in concentration range of 0.1-10.0 μg·m L-1. The intra- and inter-day RSD were3.77% and 10.60%, respectively. The average recovery was above 89.31%. Conclusion: The method is simple and rapid, which can be used for determination of paraquat in human plasma.
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