Analyze the variations of RYR2 gene and address the association with sudden cardiac death
Author(s): HAO Lu-gui, SHEN Yue-hua, Department of Investigation, Sichuan Judicial and Police Officers Professional College
Pages: 11-15
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Sudden cardiac death(SCD); RYR2; G1886S; Forensic genetics;
Abstract: Objective To analyze the variations of RYR2 gene and address the association with sudden cardiac death(SCD). Methods The genomic DNA was extracted from blood samples of the SCD group and the normal control group. The exons,exon-in-tron boundaries and 3′-UTRs of coding region of RYR2 gene were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzedstatistically. Results There were two variants in the SCD group,the latter existed certain degree difference of genotype distribution and allele frequency between the SCD groupand the control group,but there was statistically significant(P<0.05). Conclusion The gene mutation of RYR2 gene and the occurrence of Chinese SCDhave relation.
Pages: 11-15
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Sudden cardiac death(SCD); RYR2; G1886S; Forensic genetics;
Abstract: Objective To analyze the variations of RYR2 gene and address the association with sudden cardiac death(SCD). Methods The genomic DNA was extracted from blood samples of the SCD group and the normal control group. The exons,exon-in-tron boundaries and 3′-UTRs of coding region of RYR2 gene were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzedstatistically. Results There were two variants in the SCD group,the latter existed certain degree difference of genotype distribution and allele frequency between the SCD groupand the control group,but there was statistically significant(P<0.05). Conclusion The gene mutation of RYR2 gene and the occurrence of Chinese SCDhave relation.
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