Effect of EGCG on the expression of PTEN gene in the lung tissue of asthmatic mice
Author(s): YANG Nan, SHANG Yun-xiao, Department of Pediatrics, Shengjing Hospital Affiliated to China Medical University
Pages: 19-24
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Asthma; Epigallo-catechin gallate; Airway inflammation; Phosphatase and tension homology detected on chromosometen;
Abstract: Objective To investigate the effect of epigallo-catechin gallate(EGCG)on the expression of PTEN in the lung tissues in acute asthmatic mice model. Methods Thirty two healthy female BALB/c mice(Specific-pathogen-free grade,18-22 g,6-8 weeks)were randomly divided into normal control group,asthma group,EGCG(5 mg/kg)group and EGCG(50 mg/kg)group.The asthma models were established in BALB/c mice by sensitizing and challenging with ovalbumin. Airway hyperresponsiveness was detected,and lung tissues were examined for inflammatory cell infiltration by the hematoxylin and eosin staining. Serum OVA-specific Ig E level in the serum were evaluated by enzyme-linked immunosorbent assay. The expression of PTEN gene and protein in the lung tissue was measured by real-time PCR and immunohistochemistry respectively. Results Compared with normal control group,the asthma groups showed more pathological changes in the airway,higher level of OVA-s Ig E in serum and airway hyperresponsiveness to aerosolized methacholine,but lower expression of PTEN in the ling tissue(P<0.05). Low or high doses EGCG administration also significantly ameliorated inflammatory cell infiltration,reduced airway hyperresponsiveness,decreased Ig E level in the serum and incresesd the expression of PTEN in the lung tissue compared with asthma group(P<0.05). Conclusion The expression of PTEN was decre Ased in the lung tissue of the asthmatic mice,EGCG administration may play a vital role in ameliorating airway inflammation by upregulating the expression of PTEN gene.
Pages: 19-24
Year: 2016 Issue: 1
Journal: Anatomy Research
Keyword: Asthma; Epigallo-catechin gallate; Airway inflammation; Phosphatase and tension homology detected on chromosometen;
Abstract: Objective To investigate the effect of epigallo-catechin gallate(EGCG)on the expression of PTEN in the lung tissues in acute asthmatic mice model. Methods Thirty two healthy female BALB/c mice(Specific-pathogen-free grade,18-22 g,6-8 weeks)were randomly divided into normal control group,asthma group,EGCG(5 mg/kg)group and EGCG(50 mg/kg)group.The asthma models were established in BALB/c mice by sensitizing and challenging with ovalbumin. Airway hyperresponsiveness was detected,and lung tissues were examined for inflammatory cell infiltration by the hematoxylin and eosin staining. Serum OVA-specific Ig E level in the serum were evaluated by enzyme-linked immunosorbent assay. The expression of PTEN gene and protein in the lung tissue was measured by real-time PCR and immunohistochemistry respectively. Results Compared with normal control group,the asthma groups showed more pathological changes in the airway,higher level of OVA-s Ig E in serum and airway hyperresponsiveness to aerosolized methacholine,but lower expression of PTEN in the ling tissue(P<0.05). Low or high doses EGCG administration also significantly ameliorated inflammatory cell infiltration,reduced airway hyperresponsiveness,decreased Ig E level in the serum and incresesd the expression of PTEN in the lung tissue compared with asthma group(P<0.05). Conclusion The expression of PTEN was decre Ased in the lung tissue of the asthmatic mice,EGCG administration may play a vital role in ameliorating airway inflammation by upregulating the expression of PTEN gene.
Related Articles